mpm cell line 211h  (ATCC)

Journal: Scientific Reports

Article Title: Structural investigation of interactions between halogenated flavonoids and the lipid membrane along with their role as cytotoxic agents

doi: 10.1038/s41598-024-61037-y

Figure Lengend Snippet: Comparison of concentrations (µM) of compounds that inhibited 50% of the cell viability (IC 50 values) in CLBL-1, GL-1, RDSVS-TCC1, K9NK and CF2TH cells after 72 h of exposure.

Article Snippet: The CF2TH cell line was purchased from American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Comparison

Journal: International Journal of Molecular Sciences

Article Title: Hypermethylation-Mediated Silencing of CIDEA , MAL and PCDH17 Tumour Suppressor Genes in Canine DLBCL: From Multi-Omics Analyses to Mechanistic Studies

doi: 10.3390/ijms23074021

Figure Lengend Snippet: Effect of AZA + VAL or DEC + VAL combinations on CiDEA, MAL and PCDH17 protein expression in CLBL-1 cells. ( A ) Whole protein lysates from treated and untreated cells were subjected to immunoblotting, using GAPDH as the loading control. The image is representative of six independent experiments (independent cell cultures). MCF7, HepG2 and MDCK cell lines as well as CF2Th cells transfected with canine CiDEA and MAL full sequences have been used as positive controls (human and canine). Thirty and ~15 µg of total proteins were loaded in each well for the positive controls and CLBL-1 cells, respectively. ( B ) For the protein quantification of each sample, the integrated optical density (IOD) of the specific bands was normalized first to the corresponding GAPDH IOD and subsequently to the canine positive control, selected as the calibrator (CF2Th_CiDEA for CiDEA, CF2Th_MAL for MAL and MDCK for PCDH17). The results of the densitometric analysis are expressed in arbitrary units (AU) as the mean ± SEM of six independent experiments (independent cell cultures). Statistical analysis: Mann Whitney U-test (*: p < 0.05). L: ladder.

Article Snippet: Cf2Th canine normal thymus cell line obtained from European Collection of Authenticated Cell Cultures (ECACC, Porton Down, UK, Ref No. 90110521) was used for the heterologous transfection.

Techniques: Expressing, Western Blot, Control, Transfection, Positive Control, MANN-WHITNEY

Journal: International Journal of Molecular Sciences

Article Title: Hypermethylation-Mediated Silencing of CIDEA , MAL and PCDH17 Tumour Suppressor Genes in Canine DLBCL: From Multi-Omics Analyses to Mechanistic Studies

doi: 10.3390/ijms23074021

Figure Lengend Snippet: Luciferase assays of pCpGL-basic constructs containing CiDEA ( A ), MAL ( B ) and PCDH17 ( C ) CpG islands (CpGI) in CF2Th transfected cells. Luciferase activity values are expressed in arbitrary units (AU) as the fold activation relative to pCpGL-basic-mock-transfected cells (mean ± SEM of three independent experiments). On the right, a schematic diagram of CiDEA , MAL and PCDH17 gene structure and CpG-rich region position respect to the transcription starting site (TSS) is reported.

Article Snippet: Cf2Th canine normal thymus cell line obtained from European Collection of Authenticated Cell Cultures (ECACC, Porton Down, UK, Ref No. 90110521) was used for the heterologous transfection.

Techniques: Luciferase, Construct, Transfection, Activity Assay, Activation Assay

Journal: International Journal of Molecular Sciences

Article Title: Hypermethylation-Mediated Silencing of CIDEA , MAL and PCDH17 Tumour Suppressor Genes in Canine DLBCL: From Multi-Omics Analyses to Mechanistic Studies

doi: 10.3390/ijms23074021

Figure Lengend Snippet: Luciferase activity of in vitro methylated CiDEA_CpGI1 ( A ), MAL_CpGI1 ( B ), PCDH17_CpGI1 ( C ) and PCDH17_CpGI3 ( D ) plasmids in CF2Th cells. Plasmids containing the CpG sites mostly involved in the regulation of CiDEA , MAL and PCDH17 transcription were subjected to in vitro methylation. SssI, HhaI and HpaII methylation enzymes were used separately or in combination (HhaI + HpaII). Luciferase activity values (mean ± SEM of three independent experiments) are expressed as the percentage of the negative control (CTRL-, the respective unmethylated plasmid) activity, to which an arbitrary value of 100% was assigned. Statistical analysis: ANOVA + Bonferroni post hoc test (*: p < 0.05; ***: p < 0.001).

Article Snippet: Cf2Th canine normal thymus cell line obtained from European Collection of Authenticated Cell Cultures (ECACC, Porton Down, UK, Ref No. 90110521) was used for the heterologous transfection.

Techniques: Luciferase, Activity Assay, In Vitro, Methylation, Negative Control, Plasmid Preparation

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Envelope residue 375 substitutions in simian–human immunodeficiency viruses enhance CD4 binding and replication in rhesus macaques

doi: 10.1073/pnas.1606636113

Figure Lengend Snippet: SHIV Env binding to human and rhesus CD4. (A) Inhibition of SHIV entry into TZM-bl cells by human and rhesus CD4-Ig. Controls included wild-type HIV-1 Env375S expressed from infectious molecular proviral clones or as Env-pseudotyped particles. (B) SHIV entry into TZM-bl or ZB5 cells. RLU, relative light units. Variance is expressed as SD. (C) SPR analysis of HIV-1 D.191859 gp120 Env375 variants to human and rhesus CD4-Ig. Plots are of representative experiments replicated three times.

Article Snippet: The ZB5 cell line was generated as follows: Cf2Th cells (ATCC CRL-1430) were maintained in DMEM supplemented with 10% (vol/vol) FBS and 100 U/mL penicillin–streptomycin (Gibco).

Techniques: Binding Assay, Inhibition, Clone Assay